They arrive on the levels completing vats numbered 3 and you will eleven, and you may on deposit within the floor of one’s H space pavement of the Conjunto Industrial We, in addition to later versions of Keay Porcelain trials were analysed to spot amphorae articles and all-natural coatings, having fun with process designed to identify biomarkers to own wines or the derivatives, and lipid extraction to check to have lipids out-of possibly plant or animal origin which could suggest lipid-rich merchandise such organic olive oil or garum which may have already been transmitted on the ships.
An speeding up current from 15 kV was applied
The fresh new lipid removal techniques was also always characterize the new visible pitch examples. Sherds have been first facial skin-cleared up until the porcelain shot to possess analysis is actually drilled.
Powdered samples have been for every extracted using two approaches to acquisition so you’re able to accessibility lipid and mountain constituents along with you can easily biomarkers regarding drink issues. Every reagents and you may logical conditions were acquired out of Sigma Aldrich; water utilized for aqueous extractions is taken from an out in-house MilliQ plumbing related. A digital modelling exercise https://datingranking.net/nl/muslima-overzicht/ which have tungsten carbide drill parts was used. Bernal-Casasola UCA ]. New samples were after that taken off brand new bath and you will permitted to chill so you can room temperature just before getting blocked which have a good Millipore PVDF syringe filter thirteen mm diameter, 0.
The brand new removal solvent was then evaporated so you can dry skin lower than a stream away from nitrogen fuel. Contour step three – Keay 25 amphora examples regarding Algeciras The country of spain excavations D. Slope products was in fact extracted making use of the lipid removal method above that have numerous changes. Trials were upcoming placed in an ultrasonic bath to possess 10 minutes at room temperature.
Shop, derivatization in addition to introduction of your own tetratriacontane is presented according to the latest lipid extraction techniques above. The fresh new removal strategy used for the research out of wine bioples was after that removed from the new bath and you will allowed to chill in order to area temperature prior to filter because the more than.
This new aqueous removal solvent ended up being acidified so you can an excellent pH regarding as much as 2 that have hydrochloric acid. Shortly after acidification, the fresh new extraction solvent are twice drinking water-water extracted which have step 3 ml regarding ethyl acetate. The newest ethyl acetate try removed to another vial and you will evaporated so you’re able to dry skin lower than a blast of nitrogen fuel. Character of tartaric and you will syringic acids was attained by evaluation of storage moments and you can mass spectra against genuine standards also comparison contrary to the NIST11 bulk spectral databases.
Helium was applied due to the fact service provider fuel. To possess examples you to appeared as if perhaps as well concentrated so you can inject in splitless means, research was held from inside the split up function or perhaps in buy so you can supply the quantity of normal constituents before getting run in splitless form. 12 of the examples was in fact totally or nearly completely soluble from inside the this new all-natural solvent removal.
Chromatograms recognized resin acids because the found in the newest ceramic products, predominated of the dehydroabietic acid Figure 4. Other oxidation activities provided seven-oxodehydroabietic acid and you can hydroxyoxodehydroabietic acid. Heating markers including retene and you will anthracene substances had been and additionally seen on the twelve examples Colombini mais aussi al.
Pinaceae products instance abietic, pimaric and isopiples, generally speaking for the reduced density in accordance with the brand new oxidization products and every around three were not understood in every products, be open due to differing extents away from oxidation sometimes inside creation of brand new pitch otherwise through the deposition.
CLERMONT Legs 63 ASMONTFERRAND AMICAL U13
Methyl dehydroabietate has also been present in the examples, during the relatively clermont ft 63 identification quantity relative to dehydroabietic acidic. This material was indicative away from particular creation of the newest slope: it is designed because of the temperature resin-impact timber, where in fact the timber is actually burned so you can additionally extract resin from the origin timber and you may move it so you can slope Colombini mais aussi al.
