Gamma ray-giving off radionuclides in the products encapsulated on the U8 pots was indeed measured having fun with a good germanium semiconductor sensor (Canberra GC2020, GC3020 and GC4020; Mirion Technologies (Canberra) KK, Tokyo, Japan) associated with an excellent multichannel analyser.
Investigation away from physicochemical fractions
The newest physicochemical portions of 137 Cs about belly content material was in fact investigated of the classification on following around three fractions in accordance with the physicochemical portions of 137 Cs established throughout the surface: (1) an exchangeable tiny fraction; (2) a pledged in order to organic count small fraction; and you will (3) a highly bound small fraction. We presumed you to definitely (1) the newest exchangeable small fraction is bound relatively loosely which is immersed from inside the the latest gastrointestinal system; (2) this new bound to normal number fraction will natural matter which can be partly absorbed throughout digestive; and (3) the newest firmly likely small fraction is actually excreted without having to be engrossed towards the muscles.
You to definitely gram out-of dried try are placed into 10 mL off step 1 Meters ammonium nitrate service (that have a stronger–liquid ratio of 1:10) and you can prompted for 1 hours, followed closely by filtration using an excellent 0.22 ?m membrane filter out (MilliporeSigma, Burlington, MA, USA) The newest filtrate who has just the 137 Cs exchangeable fraction ended up being recovered and you can encapsulated in the an excellent U8 container.
Once modifying new good–water ratio to at least one:seven.5, 2.5 mL of 3.2 Meters ammonium acetate service (20% nitric acidic) is actually added to extract this new 137 Cs (which have a stronger–water ratio of 1:10). The latest residues was basically next added https://datingranking.net/nl/blackchristianpeoplemeet-overzicht and you will rinsed in identical matter (10 mL) from refined liquid, followed by combination towards the extraction h2o. Since the filtrate consisted of the new exchangeable and you will bound to normal number portions, the game intensity of the newest 137 Cs regarding the destined to normal matter fraction try computed of the subtracting the newest concentration of 137 Cs regarding the exchangeable small fraction (gotten into the (1)) out of that 137 Cs on filtrate.
The activity intensity of the 137 Cs in the highly-sure fraction is computed by the addition of the experience intensity of 137 Cs regarding exchangeable small fraction from inside the (1) and this in the bound to all-natural amount small fraction in (2) more than, and then subtracting that it overall regarding activity intensity of 137 Cs received to your dehydrated belly articles samples.
Mathematical investigation
I performed the statistical analyses having fun with JMP 13.dos.step one software (SAS, Cary, NC, USA). The fresh relationships between the hobby concentrations out of 137 Cs in muscle mass along with for each physicochemical tiny fraction of your own belly material (Fig. 2), the fresh new time regarding take while the interest concentration of 137 Cs inside the muscles or belly information (Fig. 3) and go out of bring and the craft concentrations away from 137 Cs of every physicochemical tiny fraction (Fig. 3) were as well as analysed. Additionally, while the region otherwise all destined to organic matter fraction is engrossed throughout the digestion, we together with analysed the full total exchangeable and destined to all-natural count fractions (Figs. dos and you can cuatro)
Matchmaking involving the hobby concentration of radiocaesium ( 137 Cs) in insane boar muscles plus some other portions from stomach information. The newest unmarried regression range formula, adjusted R-square (R dos ) and you will correlation coefficient (r) get just for the fresh new cases where a difference is actually observed between them parameters of the regression and correlation analyses. DW: Dead lbs, FM: New bulk.
Seasonal variations in the experience intensity of radiocaesium ( 137 Cs) into the crazy boar muscle mass and you may tummy content material. (a,b) Month-to-month alterations in the activity concentration of 137 Cs in muscle and tummy articles from . The top of and lower closes of field patch imply the fresh new 75th and you may 25th percentiles, correspondingly. The horizontal pub on area indicates the fresh median worth. New whiskers within higher minimizing stops of your container patch suggest the maximum and you can minimum values, respectively. (c,d) Changes in the game intensity of 137 Cs for the muscle and stomach material on the schedules if animals were caught. The fresh solitary regression line picture and you may adjusted Roentgen-rectangular (R 2 ) get only for the latest cases where a positive change is actually seen among them variables in the regression analysis. FM: Fresh size.
